GENSAT database：
GENSAT數(shù)據(jù)庫目的是把小鼠各個發(fā)育階段中樞神經(jīng)系統(tǒng)的基因定位表達并給出圖像結(jié)果；也就是可以看到不同發(fā)育階段的表達的圖像。 目前收集了三個發(fā)育期(embryonic day 15.5 (E15.5), postnatal day 7 (P7) and adult)。GENSAT本身是Gene Expression Nervous System ATlas的縮寫。實驗技術(shù)上主要是運用原位雜交和轉(zhuǎn)基因的技術(shù)；使用者可以通過查詢基因(name/ass.id等)來使用。這個數(shù)據(jù)庫是三家單位Rockefeller University，St. Jude Children's Research Hospital， NINDS主要完成的。我們可以通過[目前收集的基因]和所有數(shù)據(jù)免費下載來了解該數(shù)據(jù)庫的實際情況。下面來簡單介紹一下該數(shù)據(jù)庫的查詢方法：
The GENSAT database contains a series of images related to gene expression experiments. The images are indexed on a number of fields relevant to biological discovery. Some examples of how to search are:
1、General Entrez search help:
a、Search items are case-insensitive. 大小寫敏感
Join phrases with AND, OR, and NOT. 可以用AND/OR/NOT來組合查詢語句，但要注意這三個詞一定要大寫。
These join words are case sensitive and should be capitalized.
b、Search by gene symbol: 運用基因符號查詢
drd* This will search for all images annotated on a gene whose symbol is like 'drd'.
c、Search by gene name: 通過基因名稱查詢
dopamine This will search for all images annotated on a gene whose name contains the word 'dopamine'. 如：用"多巴胺"作為查詢詞，將查詢到基因定位圖像的注釋中包含"多巴胺“的圖像。
d、Search by mouse age: 通過小鼠的年齡查詢
In order to search by the age of the animal, the age should be followed by the word age in square brackets: dopamine AND adult[age] This will search for all images annotated on a gene whose name contains the word 'dopamine' and which were completed in an adult mouse. Currently, the database contains images obtained at the ages E10.5 (embryonic day 10.5), E15.5 (embryonic dat 15.5), P7 (post-natal day 7) and adult.
e、Search by section: 運用切片圖查詢
GENSAT images are obtained using several different procedures. In some of these procedures, the images obtained are stereotactically determined and correspond to known positions in standard neuroanatomical atlases. The sections for these follow a standard numbering from section 01 to section 11, as outlined in the GENSAT methods.
To search by section, you should append the section followed by the word 'section' in brackets: kinase AND 06[level] This searches for all images annotated on genes whose names contain the word kinase and for which an image at section '06' is available.
